B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Synonym(e): 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Empirische Formel (Hill-System): C₃₃H₃₂N₄O₈S
• Molekulargewicht: 644.69
• UNSPSC-Code: 12171500
• eCl@ss: 34058011
• PubChem Substanz-ID: 329773319
• NACRES: NA.47

Eigenschaften

• product name: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Qualitätsniveau: 100; 200
• Assay: ≥95.0% (HPLC)
• Form: powder
• Mol-Gew.: 644.71
• Farbe: light orange to very dark orange; light yellow to very dark yellow
• ε (Extinktionskoeffizient): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

Beschreibung

Anwendung

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Nützlich für die Quantifizierung von Biotin-Bindungsstellen. Dieses Reagenz bindet schneller an Avidin als herkömmliches Fluorescein-Biotin.

Biochem./physiol. Wirkung

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)