857343

Parafuchsin -hydrochlorid

certified by the Biological Stain Commission, Dye content ≥88 %

• Synonym(e): Basic Parafuchsin, Basic Red 9, Basisches Fuchsin, Magenta^™ O, Parafuchsin -hydrochlorid, Paramagenta -hydrochlorid, Pararosanilin-chlorid
• Empirische Formel (Hill-System): C₁₉H₁₇N₃ · HCl
• CAS-Nummer: 569-61-9
• Molekulargewicht: 323.82
• Farbindexnummer: 42500
• Beilstein: 4164603
• EG-Nummer: 209-321-2
• MDL-Nummer: MFCD00001657
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24888442
• NACRES: NA.47

Eigenschaften

• Qualität: certified by the Biological Stain Commission
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, ≥88%
• Farbe: green to dark green
• pH-Wert: 1.0-3.1, purple to red
• mp (Schmelzpunkt): 268-270 °C (dec.) (lit.)
• λmax: 544 nm
• ε (Extinktionskoeffizient): ≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L; ≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: [Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3
• InChI: 1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H
• InChIKey: JUQPZRLQQYSMEQ-UHFFFAOYSA-N

Beschreibung

Anwendung

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Biochem./physiol. Wirkung

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Eignung

Zertifiziert für die säurefeste Färbung mit Karbol-Fuchsin, als ENDO Medium und als Schiffs Reagens in den Periodsäure-Schiff (PAS)- und Feulgen-Techniken.

Rechtliche Hinweise

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H350
• P-Sätze: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Gefahreneinstufungen: Carc. 1B
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges