66759

Lactose-Sulfit Medium

suitable for microbiology

• Synonym(e): LS
• UNSPSC-Code: 41171607
• NACRES: NA.21

Eigenschaften

• Haltbarkeit: limited shelf life, expiry date on the label
• Zusammensetzung: Cysteine hydrochloride, 0.3 g/L ; Lactose monohydrate, 10 g/L ; Pancreatic digest of casein, 5 g/L ; Sodium chloride, 2.5 g/L ; Yeast extract, 2.5 g/L
• pH-Endwert: 7.1±0.2 (25 °C)
• Anwendung(en): microbiology

Beschreibung

Anwendung

For the confirmation of Clostridium perfringens.

Angaben zur Herstellung

Suspend 20,3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense 8 ml portions into tubes with Durham gas collecting tubes for gas detection. Sterilize in autoclave at 121 ºC for 15 minutes. Before using add to each tube 0,5 ml of a 12 g/L solution of sodium metabisulfite and 0,5 ml of a solution of 10 g/L of ferric ammonium citrate. Both solutions have to be freshly prepared and sterilized.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3