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Merck

38266

Millipore

Ferrioxamin E aus Streptomyces antibioticus

suitable for microbiology, ≥95%

Synonym(e):

1,12,23-Trihydroxy-1,6,12,17,23,28-hexaazacyclotritriacontan-2,5,13,16,24,27-hexon Eisen(III)-Komplex

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About This Item

Empirische Formel (Hill-System):
C27H45FeN6O9
CAS-Nummer:
Molekulargewicht:
653.53
MDL-Nummer:
UNSPSC-Code:
41171614
PubChem Substanz-ID:
NACRES:
NA.85

Sterilität

non-sterile

Qualitätsniveau

Assay

≥95% (HPLC)
≥95%

Form

powder

Verlust

≤6% loss on drying

Anwendung(en)

environmental
food and beverages

microbiology

Lagertemp.

2-8°C

Eignung

Salmonella spp.

SMILES String

[Fe+3].[O-]N1CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC1=O

InChI

1S/C27H45N6O9.Fe/c34-22-10-14-26(38)32(41)20-8-3-6-18-30-24(36)12-15-27(39)33(42)21-9-2-5-17-29-23(35)11-13-25(37)31(40)19-7-1-4-16-28-22;/h1-21H2,(H,28,34)(H,29,35)(H,30,36);/q-3;+3

InChIKey

MZFKJKOHYACYNT-UHFFFAOYSA-N

Allgemeine Beschreibung

Scientifically proven supplement for culture media improves detection of Salmonella, Enterobacter sakazakii and Yersinia enterocolitica .

Ferrioxamine E from Streptomyces antibioticum is a siderophore which facilitates the supply of iron (III), an essential trace element, to bacteria involved in food poisoning, including Salmonella, Enterobacter saka-zakii and Yersinia enterocolitica. It promotes rapid growth by reducing the lag phase in culture media and reactivates dormant bacteria. Use of ferrioxamine E can permit rapid detection of low bacterial cell counts, even from dry products like spices and tea after long storage periods. It is an essential component of quality control efforts in the food industry.

Ferrioxamine E is often used in Buffered Peptone Water, the medium recommended by the ISO-Norms for Enterobacteriacea. Ferrioxamine E also improves the motility of Salmonella, which helps to improve the identification by semisolid selective motility media like SIM, MRSV, DIASSALM or SMS. Ferrioxamine E does not improve growth of Escherichia coli, Shigella, Proteus, Providencia and Morganella species; a feature that makes it a semi-selective compound. The recommended concentration of Ferrioxamine E to promote bacterial growth is between 5 and 200 ng/mL
The ferrioxamines are basically metabolic products produced by the actinomycetes, and are characterised as ferrioxamines A, B, C, D 1, D2, E, F and G. Generally all ferrioxamines are trihydroxamates and the deferrioxamines are either linear or cyclic (D2 and E).

Anwendung

Der Zusatz von Ferrioxamin E in Voranreicherungsbouillons und Anreicherungsbouillons erhöht die Wiederfindungsrate von Salmonellen aus natürlich und unnatürlich kontaminierten Nahrungsmitteln signifikant.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Edward Mawji et al.
Environmental science & technology, 42(23), 8675-8680 (2009-02-06)
Siderophores are chelates produced by bacteria as part of a highly specific iron uptake mechanism. They are thought to be important in the bacterial acquisition of iron in seawater and to influence iron biogeochemistry in the ocean. We have identified
P Pless et al.
International journal of food microbiology, 27(2-3), 147-159 (1995-10-01)
Supplementation of buffered peptone water with ferrioxamine E in concentrations ranging from 0.001 to 1.0 microgram/ml significantly increased the motility of Salmonella on MSRV and DIASSALM semi-solid enrichment media. Zone diameters of swarming S. enteritidis and S. typhimurium increased more
R A Kingsley et al.
Applied and environmental microbiology, 65(4), 1610-1618 (1999-04-02)
Utilization of ferrioxamines as sole sources of iron distinguishes Salmonella enterica serotypes Typhimurium and Enteritidis from a number of related species, including Escherichia coli. Ferrioxamine supplements have therefore been used in preenrichment and selection media to increase the bacterial growth
Prakash Patel et al.
Biochemistry, 49(37), 8033-8042 (2010-08-14)
Under iron limitation, the Gram-positive bacterium Streptomyces coelicolor A3(2) excretes three siderophores of the hydroxamate type: desferrioxamine B, desferrioxamine E, and coelichelin. These sequester iron from insoluble ferric hydroxides, and the resulting ferric complexes are believed to be transported into
R Reissbrodt et al.
International journal of food microbiology, 29(1), 81-91 (1996-02-01)
Supplementation of pre-enrichment broth and enrichment broth media with ferrioxamine E (1 microgram/ml) significantly improved the recovery of Salmonella from artificially or naturally contaminated foods. Based on the selectivity of ferrioxamine E, Salmonella enteritidis and S. typhimurium could be isolated

Artikel

The expression “viable but nonculturable” (VNC) bacteria, describes cells that cannot normally be cultured; however, a better explanation of the status of these bacteria would be “not immediately culturable”.

Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.

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