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11417991001

Roche

SuRE/Cut™ Buffer H

solution, pkg of 5 × 1 mL

Synonym(e):

SuRE/Cut Buffer for Restriction Enzymes, restriction enzyme buffer

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About This Item

UNSPSC-Code:
41105600

Form

solution

Qualitätsniveau

100

Verpackung

pkg of 5 × 1 mL

Hersteller/Markenname

Roche

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

The SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.


Anwendung

SuRE/Cut™ Buffer H has been used to treat EcoRI and XhoI restriction enzymes to digest gel-purified reaction products and the pMX-IG vector. It has also been used to dilute the restriction enzyme Xanthomonas badrii I for the digestion of the genomic DNA of the E. coli colonies and the Salmonella Braenderup H2812 (ATCC BAA-664) marker.
The SuRE/Cut Buffer H has been used for digestion of genomic DNA using the restriction enzyme XbaI. It has been used for digestion of PCR products and plasmid using EcoRI and XhoI.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Rechtliche Hinweise

SuRE/Cut is a trademark of Roche

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available

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Amber E Alsop et al.
Nature communications, 6, 6841-6841 (2015-04-17)
During apoptosis, Bak permeabilizes mitochondria after undergoing major conformational changes, including poorly defined N-terminal changes. Here, we characterize those changes using 11 antibodies that were epitope mapped using peptide arrays and mutagenesis. After Bak activation by Bid, epitopes throughout the
Giel Vandemoortele et al.
Bio-protocol, 7(7), e2211-e2211 (2017-04-05)
The programmable Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease 9 (Cas9) technology revolutionized genome editing by providing an efficient way to cut the genome at a desired location (Ledford, 2015). In mammalian cells, DNA lesions trigger the error-prone non-homologous
W J M Landman et al.
Avian pathology : journal of the W.V.P.A, 43(4), 345-356 (2014-06-20)
Escherichia coli colonies isolated from the bone marrow of fresh dead hens of laying flocks with the E. coli peritonitis syndrome (EPS) were genotyped using pulsed-field gel electrophoresis (PFGE). Typing is important from an epidemiological point of view and also

Artikel

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Verwandter Inhalt

Restriction Enzymes

Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA.

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