11299964001
Roche
5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II
sufficient for ≤100 tests, storage temp.:-20°C
Synonym(e):
5-BrdU, 5-Bromo-2-deoxyuridine
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About This Item
UNSPSC-Code:
41116133
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Allgemeine Beschreibung
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.
Spezifität
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.
Anwendung
5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in:
- labeling of tooth roots for histology
- immunostaining of mice frontal sections
- immunofluorescence imaging of hepatocellular carcinoma sections
The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.
Leistungsmerkmale und Vorteile
- Safe: No radioisotopes are used.
- Easy to perform: Follows a standard immunohistochemistry protocol.
- Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
- Flexible: Allows double-labeling protocols.
Verpackung
1 kit containing 7 components.
Prinzip
Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.
Angaben zur Herstellung
Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- BrdU Labeling Reagent 1,000x concentrated
- Washing Buffer concentrate 10x concentrated
- Incubation Buffer
- Anti-BrdU antibody, contains nucleases for DNA denaturation
- Anti-mouse Ig-alkaline Phosphatase antibody
- NBT
- BCIP
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Muta. 1B - Repr. 1B - Skin Sens. 1
Lagerklassenschlüssel
3 - Flammable liquids
WGK
WGK 2
Flammpunkt (°F)
136.4 °F
Flammpunkt (°C)
58 °C
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