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10102121001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Synonym(e):

ADA, Adenosine Deaminase

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About This Item

EC-Nummer:
3.5.4.4 (BRENDA, IUBMB)
UNSPSC-Code:
12352204

Biologische Quelle

bovine intestine

Qualitätsniveau

100

Form

suspension

Spezifische Aktivität

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

Verpackung

pkg of 1 mL (10 mg)

Hersteller/Markenname

Roche

Methode(n)

activity assay: suitable

Farbe

white

Optimaler pH-Wert

7.0-7.4

Löslichkeit

water: miscible

Eignung

suitable for chemiluminescence

NCBI-Hinterlegungsnummer

AAF91430

UniProt-Hinterlegungsnummer

P56658

Anwendung(en)

life science and biopharma

Fremdaktivität

AMP-deaminase <0.01%
AP <0.01% (with pre incubation)
Guanase <0.01%
NP <0.01%

Lagertemp.

2-8°C

Angaben zum Gen

bovine ... ADA(280712)

Allgemeine Beschreibung

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Anwendung

Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Biochem./physiol. Wirkung

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Qualität

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physikalische Form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available

Analysenzertifikate (COA)

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Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
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