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TMS-008

Sigma-Aldrich

Alizarin-Red Staining Solution

Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

Synonym(e):

Alizarin Red S

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

Konzentration

40 mM in distilled water

Methode(n)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

Allgemeine Beschreibung

Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.

Anwendung

Research Category
Stammzellforschung
Research Sub Category
Osteobiologie

Qualität

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Physikalische Form

Product is presented as a 40mM solution at pH 4.1 +/- 0.1.

Lagerung und Haltbarkeit

Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Artikel

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protokolle

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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