SCT246

BioTracker BioCyTASQ G-Quadruplex (G4) Cell Probe

• Synonym(e): G quadruplex probe, G4 ligand, G4 probe, G4 tetrad probe, Live Cell Imaging Probe
• UNSPSC-Code: 41106514
• NACRES: NA.47

Eigenschaften

• Verpackung: vial of 1 mg
• Qualitätsniveau: 100
• Hersteller/Markenname: Millipore
• Wachstumsmodus: N/A
• Methode(n): DNA sequencing: suitable; RNA extraction: suitable; nucleic acid detection: suitable
• Nachweisverfahren: fluorometric
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Anwendung

G-quadruplexes are four-stranded structures comprising stacked G tetrads (G4) formed within certain guanine-rich nucleic acid sequences. G-quadruplexes are enriched in open chromatin regions but are also present in the nucleolus and cytoplasm. The formation of G4 structures is dynamic and cell-type specific, suggestive of interaction with transcription factors and other proteins. For this reason G-quadruplexes have garnered intense interest for their potential role in transcriptional regulation.

The BioTracker^™ BioCyTASQ G-quadruplex G4 cell probe is optimized for detection of G-quadruplexes. BioCyTASQ utilizes the template-assembled synthetic G-quartets (TASQ) concept as a biomimetic ligand for G-quadruplexes, a design which results in high specificity for folded G4.

References
1. Nat. Chem. 2021, 13(7): 626-633
2. ACS Chem Biol. 2021, 16(5): 905-914

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Leistungsmerkmale und Vorteile

BioCyTASQ detects ribosomal RNA associated with perinuclear rough ER regions. BioCyTASQ is suitable for G4 capture pull-down assays as well as cellular imaging.2 BioCyTASQ G4 probe is not recommended for live-cell imaging applications. The biotinylated BioTracker BioCyTASQ G-quadruplex G4 cell probe makes is a powerful tool for biochemical and cellular analysis of G4 by fluorescent detection when used with a fluorescent streptavidin.

Physikalische Form

Lyophilized. White solid.

Lagerung und Haltbarkeit

Store BioTracker BioCyTASQ G-quadruplex G4 cell probe at -20°C, desiccated and protected from light

Sonstige Hinweise

Fluorescent detection of nucleic acid G4 quadruplexes in cells and tissues

Rechtliche Hinweise

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable