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SCT214

Sigma-Aldrich

BioTracker Orange-NHS Live Cell pH dye

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.47

Qualitätsniveau

Assay

≥80% (LC)

Form

lyophilized

Methode(n)

cell based assay: suitable

Lagertemp.

−20°C

Allgemeine Beschreibung

BioTracker Orange-NHS Live Cell pH dye is a chemical probe which reversibly fluoresces upon acidic pH. The probe is modified with N-hydroxysuccimide ester (NHS) which forms a covalent bond with primary amines just by mixing. It can be used for labeling proteins, antibodies and other molecules. For example, it can be applied for visualizing endocytosis of target protein on cell membrane by labeling antibodies that binds to the target protein. This probe binds to primary amines just by mixing. Thus it can be used to label antibodies and other proteins which have lysine residues. Its fluorescence intensity increases ~20-fold at an acidic pH.

Spectral Properties
Absorbance (max): 544 nm
Emission (max): 565 nm (Orange)

Anwendung

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.
  • Cell Based Assays
  • Antibody Labeling

Verpackung

1 mg

Physikalische Form

Lyophilized

Lagerung und Haltbarkeit

Store BioTracker Orange-NHS Live Cell pH Dye at -20°C, desiccate and protect from light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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