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SCR544

Sigma-Aldrich

Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

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About This Item

UNSPSC-Code:
12352207
eCl@ss:
32161000
NACRES:
NA.71

Qualitätsniveau

100

Hersteller/Markenname

STEMCCA

Methode(n)

cell culture | stem cell: suitable

Aufnahme

sample type induced pluripotent stem cell(s)

Versandbedingung

dry ice

Allgemeine Beschreibung

The Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

Komponenten

1. Human STEMCCA Constitutive (OKSM) Lentivirus: (Part number SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Part number CS204502). Each vial contains 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.

Qualität

Tested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Yang Yu et al.
Scientific reports, 5, 10114-10114 (2015-05-13)
Human pluripotent stem cells, including cloned embryonic and induced pluripotent stem cells, offer a limitless cellular source for regenerative medicine. However, their derivation efficiency is limited, and a large proportion of cells are arrested during reprogramming. In the current study
Jun-Quan Li et al.
Molecular medicine reports, 9(3), 837-842 (2014-01-09)
Myocardial infarction (MI) is an increasing medical problem; however, its pathogenesis has yet to be elucidated and more effective treatment strategies are required. Induced pluripotent stem cells (iPSCs) were recently successfully generated using human somatic cells transfected with four transcription
Martin Madill et al.
Molecular brain, 10(1), 22-22 (2017-06-15)
Amyotrophic lateral sclerosis, a devastating neurodegenerative disease, is characterized by the progressive loss of motor neurons and the accumulation of misfolded protein aggregates. The latter suggests impaired proteostasis may be a key factor in disease pathogenesis, though the underlying mechanisms

Artikel

HumanKine® Thermostable bFGF, an engineered recombinant protein with enhanced biological functionality on human iPS and neural progenitor cells

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

Protokolle

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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