PK1109

PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: mouse, rat, chicken, human, Xenopus
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pTyr416)
• Angaben zum Gen: human ... SRC(6714)

Beschreibung

Allgemeine Beschreibung

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶.

Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶ in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.

This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src

Anwendung

Immunoblotting (1:1000)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Rekonstituierung

Do not aliquot.

Hinweis zur Analyse

Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma

Sonstige Hinweise

May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Thomas, S.M. and Brugge, J.S. 1997. Annu. Rev. Cell Dev. Biol.13, 513.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2