PC386
PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC-Code:
12352203
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
liquid
Enthält nicht
preservative
Speziesreaktivität
rat, mouse, human
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Isotyp
IgG
Versandbedingung
wet ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
phosphorylation (pSer15)
Angaben zum Gen
human ... TP53(7157)
Allgemeine Beschreibung
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
Anwendung
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Rekonstituierung
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Hinweis zur Analyse
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
Sonstige Hinweise
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
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