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Merck

PC177L

Sigma-Aldrich

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

lyophilized, Calbiochem®

Synonym(e):

Rabbit Anti-Serotonin Transporter, Serotonin Transporter Antibody

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

mouse, rat

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG

Versandbedingung

ambient

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Slc6A4(15567)

Allgemeine Beschreibung

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

Immunogen

Rat
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

Anwendung


Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Undiluted serum.

Rekonstituierung

Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

Hinweis zur Analyse

Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord

Sonstige Hinweise

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

S Nullmeier et al.
The European journal of neuroscience, 33(4), 689-698 (2011-01-14)
Heterozygous reeler mice (HRM), haploinsufficient for reelin, have been proposed to be a genetic mouse model of schizophrenia. Beside behavioural similarities, HRM also demonstrate several neuroanatomical traits similar to patients suffering from schizophrenia. In the present study using immunocytochemical procedures
Diana K Sarko et al.
The Journal of comparative neurology, 519(1), 64-74 (2010-12-02)
Northern grasshopper mice (Onychomys leucogaster) are among the most highly carnivorous rodents in North America. Because predatory mammals may have specialization of senses used to detect prey, we investigated the organization of sensory areas within grasshopper mouse neocortex and quantified
Andrea Forero et al.
Frontiers in cellular neuroscience, 11, 307-307 (2017-10-12)
Background: During early prenatal stages of brain development, serotonin (5-HT)-specific neurons migrate through somal translocation to form the raphe nuclei and subsequently begin to project to their target regions. The rostral cluster of cells, comprising the median and dorsal raphe
Rebecca L Lowery et al.
International journal of molecular sciences, 22(4) (2021-03-07)
While microglia have been established as critical mediators of synaptic plasticity, the molecular signals underlying this process are still being uncovered. Increasing evidence suggests that microglia utilize these signals in a temporally and regionally heterogeneous manner. Subsequently, it is necessary
R Ye et al.
Genes, brain, and behavior, 13(3), 261-275 (2014-01-11)
Presynaptic serotonin (5-hydroxytryptamine, 5-HT) transporters (SERT) regulate 5-HT signaling via antidepressant-sensitive clearance of released neurotransmitter. Polymorphisms in the human SERT gene (SLC6A4) have been linked to risk for multiple neuropsychiatric disorders, including depression, obsessive-compulsive disorder and autism. Using BXD recombinant

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