MABT96

Anti-Anillin Antibody, clone 5f3.1

clone 5F3.1, from mouse

• Synonym(e): Actin-binding protein anillin
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 5F3.1, monoclonal
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_061155
• UniProt-Hinterlegungsnummer: Q9NQW6
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ANLN(54443)

Beschreibung

Allgemeine Beschreibung

Anillin is a highly conserved multidomain protein that crosslinks multiple structural and regulatory elements including, but not limited to, F-actin, active myosin II, septins, and importin. It positively regulates normal cytokinesis; however, its molecular function within this context remains unclear. Failed cytokinesis results in binucleation, which can lead to genomic instability. In human cultured cells, anillin stabilizes TCA-fixable species of RhoA at the equatorial cortex, but its exact mode of action and subsequent physiological effects have yet to be determined. In some cancers, high levels of septins are shown to out-compete importin binding, thereby promoting the retention of Anillin in the cytoplasm.

Immunogen

GST-tagged recombinant protein corresponding to human Anillin.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelettale Signalübertragung

Anti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC.

Immunocytochemistry Analysis: 1 µg/mL of this antibody detected Anillin in the contractile rings of dividing HeLa cells.

Qualität

Evaluated by Western Blot in mimosine and nocodozole treated and untreated HeLa cell lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected Anillin in 10 µg of mimosine and nocodozole treated and untreated HeLa cell lysate.

Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections.

Zielbeschreibung

~160 kDa observed. The calculated molecular weight of this protein is 124 kDa, but can be observed at ~160 kDa due to high glycosylation. Uniprot describes an isoform produced by alternative splicing at ~120 kDa

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Mimosine and nocodozole treated and untreated HeLa cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable