MABT1562

Anti-CFAP43 Antibody, clone 27D5

• Synonym(e): Cilia- and flagella-associated protein 43
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 27D5, monoclonal
• Mol-Gew.: calculated mol wt 193.43 kDa; observed mol wt ~190 kDa
• Aufgereinigt durch: using protein G
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 100 μL
• Methode(n): immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2b
• Epitopsequenz: C-terminal half
• Protein-ID-Hinterlegungsnummer: NP_081835
• UniProt-Hinterlegungsnummer: E9Q7R9
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Cfap43(951475)

Beschreibung

Allgemeine Beschreibung

Cilia- and flagella-associated protein 43 (UniProt: E9Q7R9; also known as CFAP43) is encoded by the Cfap43 gene (Gene ID: 100048534) in murine species. CFAP43, a flagellar protein with six WD repeats, is expressed in testis, lung, brain, oviduct, and nasal cavity. It is detected very early during embryonic development It is involved in the regulation of the beating frequency of motile cilia on the epithelial cells. It is also involved in the formation of flagellar axoneme organization and function during spermatogenesis. Mutations in Cfap43 gene have been linked to male infertility. Any malfunction of motile cilia, resulting from defective CFAP43, cause a variety of developmental defects in humans and animal models. These defects may also include impaired mucociliary clearance of airways and hydrocephalus. Cfap43 knockout mice (-/-) exhibit dilation of the third and lateral ventricles. (Ref.: Rachev, E. (2020). Dev. Biol. 459(2); 109-125; Morimoto, Y., et al. (2019). Neurology. 92(20); e2364-e2374; Coutton, C., et al. (2018). Nature Commun. 9; Article 686).

Spezifität

Clone 27D5 is a mouse monoclonal antibody that detects Cilia- and flagella-associated protein 43. It targets an epitope within the C-terminal half.

Immunogen

MBP-tagged recombinant fragment corresponding to 174 amino acids from the C-terminal half of mouse Cilia- and flagella-associated protein 43 (CFAP43).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue extracts.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP43 in Mouse testis tissue extracts.

Tested Applications

Western Blotting Analysis: A representative lot detected CFAP43 in Western Blotting applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunofluorescence Analysis: A representative lot detected CFAP43 in Immunofluorescence applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CFAP43 in Immunoprecipitation applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Physikalische Form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Rekonstituierung

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommended storage: +2°C to +8°C.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable