MABS2049

Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1

clone 19D5.C1, from mouse

• Synonym(e): Adenylate cyclase type 5, EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 19D5.C1, monoclonal
• Speziesreaktivität: rat, porcine, mouse
• Speziesreaktivität (Voraussage durch Homologie): human (based on 100% sequence homology)
• Verpackung: antibody small pack of 25 μg
• Methode(n): ELISA: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001012783.3
• UniProt-Hinterlegungsnummer: P84309
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Adcy5(224129)

Beschreibung

Allgemeine Beschreibung

Adenylate cyclase type 5 (P84309; also known as EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5) is encoded by the Adcy5 gene (Gene ID: 224129) in murine species. Adenylate cyclase catalyzes the formation of cyclic AMP in response to G-protein signaling. It regulates the increase of free cytosolic calcium in response to increased blood glucose levels and contributes to the regulation of calcium-dependent insulin secretion. Adenylyl cyclases are typically made up of two clusters of six transmembrane domains separating two intracellular, overlapping catalytic domains. The amino acid sequence of each cytoplasmic domain that contains the nucleotide (ATP)-binding site, is well conserved among various subtypes. Thus far 9 different hormone-sensitive, membrane-bound AC isoforms have been identified. Adenylyl Cyclase 5 deficiency is shown to protect against diet-induced obesity and insulin resistance in mice and extends their life span. Adenylate cyclase 5 knockout mice display signs of reduced stress and protection against the stress of chronic pressure overload on heart. Adenylate cyclase 5 activity is stimulated by forskolin and is inhibited by adenosine and ATP analogs. (Ref.: Ho, D., et al. (2015). Diabetes. 64(7); 2636-2645. Vatner, SF., et al. (2015). Oxid. Med. Cell. Longev. 2015:250310).

Spezifität

Clone 19D5.C1 detects Adnylate Cyclase 5 in murine, rat, and procine cells. It targets an epitope within 20 amino acids from the cytoplasmic domain.

Immunogen

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to 20 amino acids from the cytoplasmic domain of murine adenylate cyclase V.

Anwendung

Research Category
Zelluläre Signaltransduktion

Anti-Adenylate Cyclase 5, clone 19D5.C1, Cat. No. MABS2049, is a mouse monoclonal antibody that detects adenylate cyclase 5 and has been tested for use in ELISA and Western Blotting.

Western Blotting Analysis: A representative lot detected Adenylyl Cyclase 5 in Western Blotting applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82; De Lorenzo, M.S., et. al. (2014). Aging Cell. 13(1):102-10).

ELISA Analysis: A representative lot detected Adenylyl Cyclase 5 in ELISA applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82).

Qualität

Evaluated by Western Blotting in mouse brain membrane preparations.

Western Blotting Analysis: 1 µg/mL of this antibody detected Adenylate Cyclase 5 in mouse brain membrane preparations.

Zielbeschreibung

~150 kDa observed; 139.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable