MABF917

Anti-CRACC Antibody, clone 4G2

clone 4G2, from rat

• Synonym(e): SLAM family member 7, CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 4G2, monoclonal
• Speziesreaktivität: mouse
• Methode(n): flow cytometry: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_653122.2
• UniProt-Hinterlegungsnummer: Q8BHK6
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Slamf7 (75345)

Beschreibung

Allgemeine Beschreibung

SLAM family member 7 (UniProt Q8BHK6; also known as CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319) is encoded by the Slamf7 (also known as Cs1) gene (Gene ID 75345) in murine species. CRACC/CS1 belongs to the 6-member (SLAM/CD150, Ly-9/CD229, 2B4/CD244, CD84, NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with each other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. CRACC is synthesized with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature CRACC with an extracellular region (a.a. 23-224) that contains a V-type and a C2-type Ig-like domain (a.a. 27-112 and 128-203), followed by a single transmembrane domain (a.a. 225-245) and a cytoplasmic tail (a.a. 246-333). CRACC cytoplasmic domain contains one immunoreceptor tyrosine-based switch motif (ITSM; 278-TFYSTV-283, numbering based on mature murine sequence), which upon phosphorylation (pY300) recruits SAP family adaptor EWS-Fli1-activated transcript-2 (EAT-2) for signaling transduction. CRACC can also be phosphorylated on Tyr280. Unlike pY300, pY280 mediates the inhibitory function of CRACC in NK cells. In addition to NK cells, evidence indicates that CRACC is also involved in regulating B- and T-cell functions.

Spezifität

Clone 4G2 reacted with murine T lymphoma BI-141 transfectants expressing mouse CRACC but not with those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305). Expected to react with all four spliced isoforms of murine CRACC reported by UniProt (Q8BHK6).

Immunogen

Epitope: Extracellular domain.

Human IgG1 Fc -tagged recombinant mouse CRACC extracellular domain.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Immunologische Signalübertragung

Anti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.

Flow Cytometry Analysis: A representative lot immunostained murine T lymphoma BI-141 transfectants expressing mouse CRACC but not those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Flow Cytometry Analysis: A representative lot detected CRACC expression on the surface of many types of mouse immune system cells, including NK cells, NKT cells, B cells, DCs, macrophages and activated T cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Affects Function: A representative lot, in combination with a rabbit anti-rat IgG, cross-linked cell surface CRACC and induced similar CRACC phosphorylation in IL-2-activated NK cells from wild-type, SAP-deficient, or EAT-2–ERT–double knock (DKO) mice (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Immunoprecipitation Analysis: A representative lot, in combination with a rabbit anti-rat IgG and protein A, immunoprecipitated CRACC from lysates of IL-2-activated mouse NK cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).

Qualität

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CRACC-positive mouse splenocytes.

Zielbeschreibung

34.77 kDa (isoform 1), 34.87 kDa (isoform 2), 30.71 kDa (isoform 3), and 30.61 kDa (isoform 4) calculated.

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG1κ antibody in PBS without preservatives.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable