MABF3091

Anti-LMP1 Antibody, clone 1G6

• Synonym(e): LMP-1, Protein p25, Protein p63
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 200
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1G6, monoclonal
• Mol-Gew.: calculated mol wt 42 kDa; observed mol wt ~N/A kDa
• Aufgereinigt durch: using protein G
• Speziesreaktivität: virus
• Verpackung: antibody small pack of 100
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2a
• Epitopsequenz: C-terminal cytoplasmic domain
• Protein-ID-Hinterlegungsnummer: N/A
• UniProt-Hinterlegungsnummer: P03230
• Lagertemp.: 2-8°C
• Angaben zum Gen: vaccinia virus ... LMP1(3783750)

Beschreibung

Spezifität

Clone 1G6 is a rat monoclonal antibody that detects LMP1. It targets an epitope within the C-terminal cytoplasmic domain of the protein.

Immunogen

Wild type LMP1-TrpE-fusion protein from Epstein-Barr virus.

Anwendung

Quality Control Testing

Evaluated by Immunocytochemistry in B95-8 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LMP1 in B95-8 cells.

Tested Applications

Immunohistochemistry Analysis: A representative lot detected LMP1 in Immunohistochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Shair, K.H.Y., et al. (2007). PLoS Pathog. 3(11):e166).

Immunocytochemistry Analysis: A representative lot detected LMP1 in Immunocytochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88).

Western Blotting Analysis: A representative lot detected LMP1 in Western Blotting applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Thornburg, N.J., et al. (2006). Oncogene. 25(2):288-97; Hannigan, A., et al. (2010). Mol Cancer. 9:9:184; Rancan, C., et al. (2015). PLoS Pathog. 11(6):e1004906).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Latent membrane protein 1 (LMP1) (UniProt: P03230) is encoded by the LMP1 (also known as BNLF1) gene (Gene ID: 3783750) in Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4). Latent membrane protein 1 (LMP1) is a viral oncoprotein encoded by the Epstein-Barr virus (EBV) and is known for its critical role in EBV-associated oncogenesis. LMP1 functions as a constitutively active mimic of the tumor necrosis factor receptor (TNFR) and can activate various signaling pathways, including NF- B, JNK, and PI3K/Akt. Through its ability to modulate these pathways, LMP1 can promote cell proliferation, inhibit apoptosis, and induce the expression of various cytokines and growth factors. Additionally, LMP1 contributes to the immortalization of infected B cells and is implicated in the pathogenesis of EBV-associated malignancies, including Hodgkin′s lymphoma and nasopharyngeal carcinoma.

Physikalische Form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstituierung

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommended storage: +2°C to +8°C.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable