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Key Documents

MABF152E

Sigma-Aldrich

Anti-F4/80 (mouse), PE, clone BM8.1 Antibody

clone BM8.1, from rat, PE

Synonym(e):

EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, F4/80

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Biologische Quelle

rat

Qualitätsniveau

Konjugat

PE

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

BM8.1, monoclonal

Speziesreaktivität

mouse

Verpackung

antibody small pack of 25 μg

Methode(n)

flow cytometry: suitable

UniProt-Hinterlegungsnummer

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Adgre1(13733)

Allgemeine Beschreibung

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Immunogen

Immunogen information for this antibody is proprietary information.

Anwendung

Research Category
Entzündung & Immunologie
Research Sub Category
Immunoglobuline & Immunologie
This rat monoclonal Anti-F4/80 Antibody (mouse), PE, clone BM8.1, Cat. No. MABF1529 is validated for use in Flow Cytometry, for the detection of F4/80.

Qualität

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected F4/80 in one million C57Bl/6 splenocytes.

Physikalische Form

Purified by size-exclusion chromatography
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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