MABE920

Anti-Mi-2β Antibody, clone 17H11

clone 17H11, from mouse, purified by affinity chromatography

• Synonym(e): Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 17H11, monoclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): ChIP: suitable (ChIP-seq); western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_001264
• UniProt-Hinterlegungsnummer: Q14839
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CHD4(1108)

Beschreibung

Allgemeine Beschreibung

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

Spezifität

Recognizes Mi-2β (CHD-4)

Immunogen

Recombinant protein corresponding to Human Mi-2β (CHD-4).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Epigenetik & nukleäre Funktionen

Research Sub Category
Chromatin-Biologie (ChIP)

Histon-modifizierende Proteine

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.

ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.

This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.

Qualität

Evaluated by Western Blotting in a Jurkat cell lystate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.

Zielbeschreibung

~ 220 kDa observed

Physikalische Form

Protein G Purified

Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable