MABE366

Anti-SNF2L Antibody, clone SNF 2C4

clone SNF 2C4, from rat

• Synonym(e): Probable global transcription activator SNF2L1, ATP-dependent helicase SMARCA1, Nucleosome-remodeling factor subunit SNF2L, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: SNF 2C4, monoclonal
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_003060
• UniProt-Hinterlegungsnummer: P28370
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SMARCA1(6594)

Beschreibung

Allgemeine Beschreibung

Nucleosome-remodeling factor subunit SNF2L, also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1 (SMARCA1), is the energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. These complexes facilitate the disruption of chromatin structure in an ATP-dependent manner. SNF2L potentiates neurite outgrowth, and may be involved in brain development by regulating En-1 and En-2 expression as well as in the development of luteal cells.

Immunogen

GST-tagged recombinant protein corresponding to human SNF2L.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Chromatin-Biologie (ChIP)

Detect the SNF2L protein using this Anti-SNF2L, clone SNF 2C4 validated for use in WB & IHC.

Western Blot Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in untransfected HeLa whole cell extracts, but demonstrated a loss of signal in HeLa whole cell extracts transfected with siRNA. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in certain, normal human organ tissues. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).

Qualität

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected SNF2L in 10 µg of HeLa cell lysate.

Zielbeschreibung

~121 and ~123 kDa observed. Uniprot describes 5 tyrosine phosphorylation sites.

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HeLa cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable