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MABE1015

Sigma-Aldrich

Anti-CST complex subunit CTC1 Antibody, clone 8E5.2

clone 8E5.2, from mouse

Synonym(e):

CST complex subunit CTC1, Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

8E5.2, monoclonal

Speziesreaktivität

human

Methode(n)

western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CTC1(80169)

Allgemeine Beschreibung

CST complex subunit CTC1 (UniProt Q2NKJ3; also known as Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B) is encoded by the CTC1 (also known as C17orf68, CRMCC) gene (Gene ID 80169) in human. CTC1 is a subunit of the trimeric CST (CTC1, STN1, and TEN1) complex that binds ssDNA, promotes telomere DNA synthesis, and inhibits telomerase-mediated telomere elongation. CST directly interacts with and stimulates DNA polymerase α-primase (polα-primase) activity. CST also associates with and sequesters telomeric 3′ overhangs after their elongation by telomerase, thereby limiting further telomere extension by telomerase. Knockdown of either STN1 or CCT1 in human cancer cells and in mouse fibroblasts results in substantially elongated telomeric G-overhangs. Conditional deletion of CTC1 in mice leads to early entry into senescence and signs of telomeric replication defects, Human CTC1 gene mutations casue dyskeratosis congenita (DC) and related bone marrow failure syndromes, as well as the rare autosomal recessive neurological disorder Coats Plus, where patients develop cranial calcifications and cysts. Coats Plus and DC patients develop premature hair graying, anemia, and osteoporosis as a result of telomerase defects.

Spezifität

Expected to react with both spliced isoforms of human CTC1 reported by UniProt (Q2NKJ3).

Immunogen

Epitope: Internal (C-terminal half).
GST-tagged recombinant internal fragment from the C-terminal half of human CTC1.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur
Anti-CST complex subunit CTC1 Antibody, clone 8E5.2 is an antibody against CST complex subunit CTC1 for use in Western Blotting.

Qualität

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CST complex subunit CTC1 in 10 µg of human cortex tissue lysate.

Zielbeschreibung

~135 kDa observed. 134.6 kDa (isoform 1) and 114.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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