MABE1012

Anti-E2F-7 Antibody, clone 14C3.2

clone 14C3.2, from mouse

• Synonym(e): Transcription factor E2F7, E2F-7
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 14C3.2, monoclonal
• Speziesreaktivität: mouse, human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_976328
• UniProt-Hinterlegungsnummer: Q96AV8
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... E2F7(144455)

Beschreibung

Allgemeine Beschreibung

Transcription factor E2F7 (UniProt Q96AV8; also known as E2F-7) is encoded by the E2F7 gene (Gene ID 144455) in human. The E2F family of transcription factors (E2F) regulate genes involved in cell proliferation, differentiation and apoptosis. E2F1, 2, 3a function as transcription activators, while other family members (E2F3b, 4, 5, 6, 7, 8) are known as transcription repressors. Upregulated E2F7 is found in tamoxifen-resistant breast cancer cells, where it is responsible for transcriptional repression of miR-15a/16. Exogenous expression of miR-15a/16 or silencing E2F7 re-sensitizes breast cancer cells to tamoxifen-induced cell cycle arrest and apoptosis. Upregulated E2F7 expression is also reported in cutaneous squamous cell carcinomas (CSCC) and and head and neck SCC (HNSCC), where E2F7-dependent Sphk1 overexpression in SCC results in an elevated production of the antiapoptotic phospholipid, sphingosine-1-phosphate (S1P), which in turn invokes anthracycline resistance via activation of the PI3K/AKT pathway.

Spezifität

Clone 14C3.2 recognizes an internal epitope of human E2F-7 present in all three spliced isoforms reported by UniProt (Q96AV8).

Immunogen

Epitope: Internal (C-terminal half).

GST-tagged recombinant human E2F-7 internal fragment.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected E2F-7 in A431, HeLa, HepG2 and NIH/3T3 cells.

This Anti-E2F-7 Antibody, clone 14C3.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of E2F-7.

Qualität

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected E2F-7 in 10 µg of HEK293 cell lysate.

Zielbeschreibung

~100 kDa observed. 99.89 kDa (isoform 1; E2F7b), 80.87 kDa (isoform 2; E2F7a), 98.35 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable