MABD122

Anti-CD73 Antibody, clone AA60-E3-3

clone AA60-E3-3, from mouse

• Synonym(e): 5′-nucleotidase, 5′-NT, ecto-5′-nucleotidase
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: AA60-E3-3, monoclonal
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): mouse (immunogen homology)
• Methode(n): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001191742
• UniProt-Hinterlegungsnummer: P21589
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NT5E(4907)

Beschreibung

Allgemeine Beschreibung

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Mesenchymale Stammzellen

Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.

This antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.

Qualität

Evaluated by Flow Cytometry on human adipose mesenchymal stem cells.
Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.

Zielbeschreibung

63 kDa calculated

Verlinkung

Replaces: MABC668

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Human Adipose Mesenchymal Stem Cells

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable