MABC573

Anti-Bcl-2 Antibody, clone 1D7.2

clone 1D7.2, from rat

• Synonym(e): Apoptosis regulator Bcl-2, Bcl-2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 1D7.2, monoclonal
• Speziesreaktivität: mouse
• Methode(n): western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_033871
• UniProt-Hinterlegungsnummer: P10417
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Bcl2(12043)

Beschreibung

Allgemeine Beschreibung

Apoptosis regulator Bcl-2 (UniProt P10417; also known as B-cell leukemia/lymphoma 2) is encoded by the Bcl2 (also known as Bcl-2, AW986256, C430015F12Rik, D630044D05Rik, D830018M01Rik) gene (Gene ID 12043) in murine species. Bcl-2 is an anti-apoptotic and the founding member of the Bcl-2 family of proteins that paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1–BH4), whereas the BH3-only members contain only the BH3 amphipathic helix, which mediates their interaction with the groove of multi-domain BCL-2 family members. Human BCL2 gene mutatons are well known causes of many cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer.

Spezifität

Expected to react with both spliced isoforms of murine Bcl-2.

Immunogen

Epitope: N-terminal half

GST-tagged recombinant protein corresponding to the N-terminal of mouse Bcl-2.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
Apoptose - Weiterführende Produkte

Detect Bcl-2 using this rat Monoclonal Anti-Bcl-2 Antibody, clone 1D7.2, Cat. No. MABC573, validated for use in Western Blotting.

Qualität

Evaluated by Western Blotting in mouse myoblast C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Bcl-2 in 10 µg of mouse myoblast C2C12 cell lysate.

Zielbeschreibung

~22 kDa observed (26.4 kDa/isoform 1 & 22.3 kDa/isoform 2, calculated).

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable