MABC44

Anti-LAMP-3 Antibody, clone 16H11.2

clone 16H11.2, from mouse

• Synonym(e): LAMP, CD208, LAMP-3, DC-loysosome-associated membrane glycoprotein, DC LAMP, DC-LAMP, Protein TSC403
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 16H11.2, monoclonal
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_055213
• UniProt-Hinterlegungsnummer: Q9UQV4
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LAMP3(27074)

Beschreibung

Allgemeine Beschreibung

Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.

Immunogen

Recombinant protein corresponding to human LAMP-3.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Apoptose - Weiterführende Produkte

Organell- & Zellmarker

Detect LAMP-3 using this Anti-LAMP-3 Antibody, clone 16H11.2 validated for use in WB, IH.

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected LAMP-3 in colorectal adenocarcinoma tissue.

Qualität

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-3 in MCF-7 cell lysate.

Zielbeschreibung

~65 kDa observed.
The calculated molecular weight of this protein is 41 kDa, but has been observed at ~65-90 kDa due to glycosolation (de Saint-Vis, B., et al. (1998). Immunity. 9(3):325-336).

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
MCF-7 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable