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Key Documents

MAB5446

Sigma-Aldrich

Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24

ascites fluid, clone RMO-24, Chemicon®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

RMO-24, monoclonal

Speziesreaktivität

human, rat

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... NEFH(4744)
rat ... Nefh(24587)

Spezifität

Reacts with Neurofilament Heavy (NF-H), tail domain (phosphate dependent). Dephosphorylation of the NF-H will results in diminished immunoreactivity.

Immunogen

Epitope: tail domain (Phosphate dependent)
Rat Neurofilaments

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Neurofilament & Neuronen-Stoffwechsel

Neuronen- & Gliamarker
Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 detects level of Neurofilament NF-H & has been published & validated for use in ELISA, IP, WB, IH.
Western blot. Reacts with the 200 kD NF-H from human.

Immunohistochemistry. The antibody has been used successfully on Bouin′s and alcohol-fixed paraffin-embedded or frozen tissue sections. Formalin-fixed paraffin-embedded tissue sections require pretreatment with trypsin.

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Physikalische Form

Ascites fluid. Liquid.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
Positive Control: Brain

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Hirofumi Tokuoka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(45), 11606-11614 (2006-11-10)
The mechanism by which synaptic vesicles (SVs) are recruited to the release site is poorly understood. One candidate mechanism for trafficking of SVs is the myosin-actin motor system. Myosin activity is modulated by myosin light chain kinase (MLCK), which in
V M Lee et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3474-3488 (1987-11-01)
A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and

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