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Key Documents

MAB5210

Sigma-Aldrich

Anti-Phosphacan Antibody, clone 122.2

ascites fluid, clone 122.2, Chemicon®

Synonym(e):

Receptor Tyrosine Phosphatase beta

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

122.2, monoclonal

Speziesreaktivität

rat

Hersteller/Markenname

Chemicon®

Methode(n)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotyp

IgM

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PTPRZ1(5803)

Allgemeine Beschreibung

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

Spezifität

Phosphacan (Receptor Tyrosine Phosphatase Beta). Recognizes the core protein.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Wachstumskegel & Axonlenkung
Anti-Phosphacan Antibody, clone 122.2 detects level of Phosphacan & has been published & validated for use in WB, IC, IH.
Western blot. Recognizes bands at 250, 190, and 180 kDa on western blots of embryonic rat brain extracts.

Immunocytochemistry: 1:10

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Physikalische Form

Ascites fluid. Liquid. Contains no preservative.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
POSITIVE CONTROL:

embryonic or early post-natal rat brain.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Progressive changes in adherens junction structure during intestinal adenoma formation in Apc mutant mice.
Carothers, AM; Melstrom, KA; Mueller, JD; Weyant, MJ; Bertagnolli, MM
The Journal of Biological Chemistry null
Wu-Fu Chen et al.
CNS neuroscience & therapeutics, 21(9), 698-707 (2015-07-21)
To date, no reliable methods have proven effective for treating spinal cord injury (SCI). Even systemic administration of methylprednisolone (MP) remains controversial. We previously reported that intrathecal (i.t.) administration of granulocyte colony-stimulating factor (G-CSF) improves outcome after experimental spinal cord
Bala T S Susarla et al.
Journal of neurochemistry, 119(4), 868-878 (2011-09-08)
Traumatic injury to the CNS results in increased expression and deposition of chondroitin sulfate proteoglycans (CSPGs) that are inhibitory to axonal regeneration. Transforming growth factor-β (TGF-β) has been implicated as a major mediator of these changes, but the mechanisms through
Marc R Del Bigio et al.
Cerebrospinal fluid research, 5, 12-12 (2008-07-12)
The cerebral cortex may be compressed in hydrocephalus and some experiments suggest that movement of extracellular substances through the cortex is impaired. We hypothesized that the extracellular compartment is reduced in size and that the composition of the extracellular compartment
Helen B Treloar et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(30), 9405-9416 (2009-07-31)
We recently described the boundary-like expression pattern of the extracellular matrix molecule tenascin-C (Tnc) in the developing mouse olfactory bulb (OB) (Shay et al., 2008). In the present study, we test the hypothesis that Tnc inhibits olfactory sensory neuron (OSN)

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