MAB4349

Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

clone TRA-2-49/6E, Chemicon^®, from mouse

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: TRA-2-49/6E, monoclonal
• Speziesreaktivität: rabbit, feline, pig, human
• Darf nicht reagieren mit: rat, monkey, guinea pig, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable
• Aufnahme: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• Isotyp: IgG1
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Spezifität

Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.

Immunogen

2102Ep human Embryonal Carcinoma Cells

Epitope: Liver/Bone/Kidney Alkaline Phosphatase

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase

Immunocytochemistry: A previous lot was used on IC.

Immunofluorescence: A previous lot of this antibody was used in IF.

FACS Analysis: A starting range of 5-20 µg/mL is suggested.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by end user.

This Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E is validated for use in FC, IF, IC, IP for the detection of TRA-2-49.

Zielbeschreibung

212/80/55 kDa

Physikalische Form

Protein A purified

Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide

Lagerung und Haltbarkeit

Stable for 1 year at from date of receipt.

Hinweis zur Analyse

Control
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable