MAB3582

Anti-Fascin Antibody, clone 55K2

clone 55K2, Chemicon^®, from mouse

• Synonym(e): Anti-FAN1, Anti-SNL, Anti-p55
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 55K2, monoclonal
• Speziesreaktivität: echinoderm, sea urchin, gerbil, human
• Speziesreaktivität (Voraussage durch Homologie): mammals
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG1
• Eignung: not suitable for immunoprecipitation
• NCBI-Hinterlegungsnummer: NM_003088.2
• UniProt-Hinterlegungsnummer: Q16658
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FSCN1(6624)

Beschreibung

Spezifität

Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.

Immunogen

Human fascin

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelett

This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.

Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Format: Purified

Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.

Hinweis zur Analyse

Control
POSITIVE CONTROL:

Hela cells, neuronal tissues, antigen presenting dendritic cells.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable