MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
ascites fluid, clone 2HP-2G9, Chemicon®
Synonym(e):
HP1a, Antigen p25, Chromobox Protein Homolog 5
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
ascites fluid
Antikörper-Produkttyp
primary antibodies
Klon
2HP-2G9, monoclonal
Speziesreaktivität
mouse, human
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG1
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... CBX5(23468)
Allgemeine Beschreibung
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Spezifität
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
Immunogen
Recombinant mouse HP1alpha.
Anwendung
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Zielbeschreibung
25 kDa
Physikalische Form
Liquid ascites fluid containing no preservative.
Hinweis zur Analyse
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Up-regulation of Rac GTPase activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Wang SM, Ooi LL, Hui KM
Clinical cancer research : an official journal of the American Association for Cancer Research null
Akinori Takahashi et al.
Experimental cell research, 315(7), 1117-1141 (2009-02-28)
Src-family kinases (SFKs), which participate in various signaling events, are found at not only the plasma membrane but also several subcellular compartments, including the nucleus. Nuclear structural changes are frequently observed during transcription, cell differentiation, senescence, tumorigenesis, and cell cycle.
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.
Terada, Y
Molecular Biology of the Cell null
A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.
Obuse, C; Iwasaki, O; Kiyomitsu, T; Goshima, G; Toyoda, Y; Yanagida, M
Nature Cell Biology null
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Nath DS, Tiriveedhi V, Basha HI, Phelan D, Moazami N, Ewald GA, Mohanakumar T.
Transplantation null
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