MAB1596

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

clone 6G6-1C9, Chemicon^®, from mouse

• Synonym(e): PSD-95
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 6G6-1C9, monoclonal
• Speziesreaktivität: mouse, rat, bovine
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2a
• NCBI-Hinterlegungsnummer: NM_001128827.1
• UniProt-Hinterlegungsnummer: P78352
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: bovine ... Dlg4(100137840) ; mouse ... Dlg4(13385) ; rat ... Dlg4(29495)

Beschreibung

Allgemeine Beschreibung

PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.

Spezifität

Other species have not been tested.

Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.

Immunogen

Recombinant rat PSD-95.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Synapsen & Synapsenbiologie

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.

Immunocytochemistry:
12.5 µg/mL

Western Blot:
1-5 μg/mL (ECL) on rat brain lysate (20 μg).

Optimal working dilutions must be determined by the end user.

Qualität

Routinely evaluated in Immunocytochemistry.

Zielbeschreibung

~100 kDa

Verlinkung

Replaces: 04-1066

Physikalische Form

See datasheet for purification method.

Format: Purified

Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.

Lagerung und Haltbarkeit

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Hinweis zur Analyse

Control
Mouse brain, rat brain tissue.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2