IPFL10100

Immobilon^® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

• Synonym(e): Western blotting membrane, blotting membrane, transfer membrane
• UNSPSC-Code: 41105339
• eCl@ss: 32031602
• NACRES: NB.22

Eigenschaften

• product name: Immobilon^®-FL PVDF Membrane, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
• Materialien: PVDF membrane; plain filter; white filter
• Qualitätsniveau: 400
• Leistungsmerkmale: hydrophobic
• Hersteller/Markenname: Immobilon^®
• Methode(n): dot blot: suitable; western blot: suitable
• Filter L × B: 10 cm × 10 cm
• Porengröße: 0.45 μm pore size
• Kapazität: 155 μg/cm² adsorption capacity (insulin); 205 μg/cm² adsorption capacity (BSA); 300 μg/cm² adsorption capacity (goat IgG)
• Kompatibilität: for use with Amido black; for use with CPTS; for use with Coomassie brilliant blue; for use with Ponceau-S red
• Nachweisverfahren: chemiluminescent; colorimetric; fluorometric
• Versandbedingung: ambient

Beschreibung

Allgemeine Beschreibung

The Immobilon^®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon^®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Anwendung

Immobilon^®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.

Leistungsmerkmale und Vorteile

Immobilon^®-FL Membrane:

• The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
• Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
• compatible with standard blocking agents and buffers
• Ideal for multiplexing and chemifluorescence detections

Rechtliche Hinweise

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable