GF134

Pigment Epithelium Derived Factor Protein, Recombinant human

The Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991).

• Synonym(e): PEDF
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Assay: >95% (SDS-PAGE)
• Form: liquid
• Hersteller/Markenname: Chemicon^®
• NCBI-Hinterlegungsnummer: NM_002615.4
• UniProt-Hinterlegungsnummer: P36955
• Versandbedingung: dry ice

Beschreibung

Allgemeine Beschreibung

Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991). PEDF has been shown to have neurotrophic activity in the retina in vivo (Cayouette et al., 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, et al., 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson et al., 2001; Stellmach et al., 2001).

Product Source: Stable BHK (baby hamster kidney) cell transfectants

Physikalische Form

Provided liquid in 20 mM sodium phosphate, pH 6.4, 0.2 M sodium chloride, and 1 mM DTT. Product has been sterile filtered to 0.22-micron.

Angaben zur Herstellung

Product may be rendered sterile by 0.22-micron filtration.

Lagerung und Haltbarkeit

Maintain frozen at -70°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

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Hinweis zur Analyse

PEDF is purified via affinity purification from culture supernatants

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable