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Merck

F1904

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, purified 120 kDa α Chymotryptic Fragment (cell attachment region), suitable for cell culture

Synonym(e):

Fibronectin Fragment, Human Fibronectin

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About This Item

UNSPSC-Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified,

Biologische Quelle

human

Qualitätsniveau

Form

lyophilized

Mol-Gew.

120 kDa

Hersteller/Markenname

Chemicon®

Konzentration

1 mg/mL

Methode(n)

cell culture | mammalian: suitable

Aufnahme

sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... FN1(2335)

Allgemeine Beschreibung

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.
Product Source: Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Anwendung

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Physikalische Form

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

Lagerung und Haltbarkeit

Maintain material at -20°C for 3 months or -70°C for six months.

Hinweis zur Analyse

One major band at 120kDa in SDS-PAGE

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Kunden haben sich ebenfalls angesehen

Fibronectin: purification, immunochemical properties, and biological activities.
Ruoslahti, E, et al.
Test, 82 Pt A, 803-831 (1982)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

Artikel

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protokolle

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Verwandter Inhalt

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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