CP70
Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
liquid, clone FBN11, Calbiochem®
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About This Item
UNSPSC-Code:
12352203
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
FBN11, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität
rat, human, mouse
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
do not freeze
Isotyp
IgG1
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)
Allgemeine Beschreibung
Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
Immunogen
recombinant human fibronectin
Anwendung
Frozen Sections (0.5-1 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Immunoblotting (0.5-1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Hinweis zur Analyse
Positive Control
SW 156 cells or normal kidney tissue
SW 156 cells or normal kidney tissue
Sonstige Hinweise
Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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