BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem^®

• UNSPSC-Code: 41116006

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: Novabiochem^®
• Form: liquid
• Eignung der Reaktion: reaction type: Coupling Reactions; reagent type: catalyst
• Übergangstemp.: flash point 2 °C (Acetonitrile)
• Versandbedingung: ambient
• Lagertemp.: 2-30°C

Beschreibung

Allgemeine Beschreibung

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

Anwendung

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

Leistungsmerkmale und Vorteile

• efficient and rapid activation reagent that produces less
• phosphoramidite waste      
• higher solubility at lower temperatures      
• does not crystallize or clog lines       
• safer reagent to use

Hinweis zur Analyse

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

Rechtliche Hinweise

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS02,GHS07
• Signalwort: Danger
• H-Sätze: H225 - H302 + H312 + H332 - H319
• P-Sätze: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 3
• Flammpunkt (°F): 35.6 °F - (Acetonitrile)
• Flammpunkt (°C): 2 °C - (Acetonitrile)