ABT319

Anti-Nephrin Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Nephrin, Renal glomerulus-specific cell adhesion receptor
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_004637
• UniProt-Hinterlegungsnummer: O60500
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NPHS1(4868)

Beschreibung

Allgemeine Beschreibung

Nephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

Spezifität

This antibody is expected to detect both known isoforms of Nephrin.

Immunogen

Epitope: NPHS2 binding domain

KLH-conjugated linear peptide corresponding to the NPHS2 binding domain of human Nephrin.

Anwendung

Research Category
Zellstruktur

Research Sub Category
ECM-Proteine

Anti-Nephrin Antibody is a highly specific rabbit polyclonal antibody, that targets Nephrin & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Nephrin in human kidney tissue.

Qualität

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nephrin in 10 µg of human kidney tissue lysate.

Zielbeschreibung

~190 kDa and ~85 kDa observed. Two isoforms are known to exist at 134kDa (Isoform 1) and 130kDa (Isoform 2). This protein may be observed at higher molecular weights due to glycosylation (Ruotsalainen, V., et al. (1999). Proc Natl Acad Sci USA. 6(96):7962-7967.).

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable