ABT119

Anti-CELSR1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Cadherin EGF LAG seven-pass G-type receptor 1, Cadherin family member 9, Flamingo homolog 2, hFmi2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology), human (based on 100% sequence homology)
• Methode(n): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_055061
• UniProt-Hinterlegungsnummer: Q9NYQ6
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CELSR1(9620)

Beschreibung

Allgemeine Beschreibung

The Flamingo/CELSR family of 7TM-cadherins are characterized with a symmetric protein distribution and polarized activity at neighboring epithelial cell interfaces along defined axes of planar cell polarity. As a component of the extracellular matrix, the CELSR1 protein has been found particularly at the basal surface of neuroepithelial cells within both the early neural tube and a less well-defined group of ventricular zone cells at the midline of the developing spinal cord. CELSR1 plays a role in orienting hair follicles along the anterior–posterior axis of the developing mouse epidermis, both during embryogenesis and postnatal development.

Immunogen

Linear peptide corresponding to human CELSR1.

Anwendung

Immunofluorescence Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected CELSR1 in rat cerebellum, substancia nigra, and hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected CELSR1 in neurons of rat cortex tissue and in Purkinje cells and cells within the granular layer of rat cerebellum tissue.

This Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.

Qualität

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CELSR1 in 10 µg of PC12 cell lysate.

Zielbeschreibung

~330 kDa observed. A second isoform at ~152 kDa may be observed in some lysates.
An uncharacterized band at ~54 kDa may be observed in some cell lysates.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable