ABS1515

Anti-phospho NFkB p52, (Ser222) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, Nuclear factor NF-kappa-B p52 subunit
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002493
• UniProt-Hinterlegungsnummer: Q00653
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer222)
• Angaben zum Gen: human ... NFKB2(4791)

Beschreibung

Allgemeine Beschreibung

The p52 NF-kB subunit is induced through activation of the alternative (noncanonical) NF-kB pathway in response to a range of stimuli such as lymphotoxin-b, BAFF and CD40, as well as DNA damage. The p52 formation results from proteolytic processing of its precursor protein p100 as a consequence of IKKalpha-mediated phosphorylation. The p52 is most commonly found as a heterodimer with RelB, although other dimer combinations have been identified by ChIP analysis. Ser222 of NF-kappa B p52 is phosphorylated by the kinase GSK3beta. Phosphorylation at S222 appears to regulate not only p52 partner dimerization but also the p52 dependent activation or repression of Skp2 gene expression.

Immunogen

Epitope: RHD domain

KLH-conjugated linear peptide corresponding to the RHD domain of human phospho NFkB p52, (Ser222).

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Signalübertragung & Neurowissenschaft

Anti-phospho NFkB p52, (Ser222) is an antibody against phospho NFkB p52 for use in Western Blotting.

Western Blotting Analysis: A representative lot detected phospho NFkB p52, (Ser222) in CD40L and NTL cell lysate (Neil D. Perkins, Newcastle University).

Qualität

Evaluated by Western Blotting in cell lysate U2OS transfected with p52 WT.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho NFkB p52, (Ser222) in 10 µg of cell lysate U2OS transfected with p52 WT.

Zielbeschreibung

~52 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1