Direkt zum Inhalt
Merck
Alle Fotos(2)

Key Documents

ABS1006

Sigma-Aldrich

Anti-PCSK9 Antibody

from rabbit, purified by affinity chromatography

Synonym(e):

NARC-1, PC9, Subtilisin/kexin-like protease PC9, Proprotein convertase 9, Neural apoptosis-regulated convertase 1, Proprotein convertase subtilisin/kexin type 9

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

human

Methode(n)

immunocytochemistry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PCSK9(255738)

Allgemeine Beschreibung

PCSK9 protein is an important protein in the regulation of plasma cholesterol homeostasis. PCSK9 binds various lipid receptor protein members including low density lipoprotein receptors (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2) and promotes their degradation or sequestration. In neurons, PCSK9 regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. PCSK9 is localized in cytoplasm but it is also secreted and thus can be found in endosomes, lysosomes, and endoplasmic reticulum and Golgi as well. PCSK9 is expressed in normal cells such as Schwann cells and pancreatic epithelial cells but PCSK9 is also expressed in many cancers and cancer cells lines as well.

Immunogen

His-tagged recombinant protein corresponding to Human PCSK9.

Anwendung

Research Category
Zelluläre Signaltransduktion

Stoffwechsel
Research Sub Category
Lipidabhängige Signalübertragung

Leberphysiologie
This Anti-PCSK9 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of PCSK9.
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in treated HepG2 cell lysate (Jeong, H. J., et al. J. Lipid Res. 2008. 49: 399–409.)
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in siRNA transfected HepG2 cells without and with BBR treatment (Data compliments of Sahng Wook Park of Yongsei University College of Medicine).

Qualität

Evaluated by Immunocytochemistry on HepG2 cells
Immunocytochemistry Analysis: 8.0 µg/mL of this antibody detected PCSK9 in HepG2 cells.

Zielbeschreibung

~75 kDa observed

Physikalische Form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Sterol-dependent regulation of proprotein convertase subtilisin/kexin type 9 expression by sterol-regulatory element binding protein-2.
Jeong, HJ; Lee, HS; Kim, KS; Kim, YK; Yoon, D; Park, SW
Journal of Lipid Research null
Hepatocyte nuclear factor 1alpha plays a critical role in PCSK9 gene transcription and regulation by the natural hypocholesterolemic compound berberine.
Li, H; Dong, B; Park, SW; Lee, HS; Chen, W; Liu, J
The Journal of Biological Chemistry null
Strong induction of PCSK9 gene expression through HNF1alpha and SREBP2: mechanism for the resistance to LDL-cholesterol lowering effect of statins in dyslipidemic hamsters.
Dong, B; Wu, M; Li, H; Kraemer, FB; Adeli, K; Seidah, NG; Park, SW; Liu, J
Journal of Lipid Research null

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.