ABE30

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

from rabbit, purified by affinity chromatography

• Synonym(e): polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat (based on 100% sequence homology), horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_000928
• UniProt-Hinterlegungsnummer: P24928
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... POLR2B(5431)

Beschreibung

Allgemeine Beschreibung

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RPB1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

Immunogen

KLH-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
RNA-Stoffwechsel & Bindungsproteine

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RNA polymerase II subunit B1 in HeLa cells.

Use Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 (rabbit polyclonal antibody) validated in WB, ICC to detect RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II subunit RPB1.

Qualität

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected RNA polymerase II subunit B1 on 10 µg of HeLa nuclear extract.

Zielbeschreibung

~217 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HeLa nuclear extract

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable