ABE1828

Anti-FOXK2 Antibody

serum, from rabbit

• Synonym(e): Forkhead box protein K2, Cellular transcription factor ILF-1, FOXK1, Interleukin enhancer-binding factor 1, FOXK2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: serum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_004505
• UniProt-Hinterlegungsnummer: Q01167
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FOXK2(3607)

Beschreibung

Allgemeine Beschreibung

Forkhead box protein K2 (UniProt Q01167; also known as FOXK2, Cellular transcription factor ILF-1, Interleukin enhancer-binding factor 1) is encoded by the FOXK2 (also known as ILF, ILF-1, ILF1) gene (Gene ID 3607) in human. FOXK2 is a transcription factor involved in cell proliferation and survival. FOXK1 and FOXK2 belong to the family of Fox transcription factors characterized by the forkhead DNA-binding domains. However, FOXK1 and FOXK2 can be distinguished from other Fox transcription factors by the presence of the forkhead-associated (FHA) domains. FOXK2 interacts with BRCA1-associated protein 1 (BAP1) through the FHA domain in a BAP1 Thr493 phosphorylation-dependent manner. BAP1 in turn recruits HCF-1, thereby forming a ternary complex at the FOXK2 target genes sites, where BAP1 represses FOXK2 target genes expression in a BAP1 DUB activity-dependent manner.

Spezifität

Expected to react with isoforms 1&2, but not isoform 3.

Immunogen

Epitope: C-terminal

Recombinant protein corresponding to the C-terminal region of human FOXK2.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Immunoprecipitation Analysis: A representative lot immunoprecipitated FOXK2 in HeLa nuclear extract (Data courtesy of El Bachir Affar, University of Montreal).
Western Blotting Analysis: A representative lot detected FOXK2 in tag-affinity purified BAP1 complex from HeLa cells expressing Flag-HA-tagged wild-type BAP1 or catalytically dead C91S BAP1 mutant (Mashtalir, N., et al. (2014). Mol. Cell. 54(3):392-406).

Research Sub Category
Nuclear Receptors

This Anti-FOXK2 Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of FOXK2 .

Qualität

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FOXK2 in 10 µg of HeLa nuclear extract.

Zielbeschreibung

~78 kDa observed. Isoform 1 (69.1 kDa calculated); Isoform 2 (64.3 kDa calculated).

Physikalische Form

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1