ABC955

Anti-GADD153/DDIT3/CHOP Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, GADD153/DDIT3/
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): monkey (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_004074
• UniProt-Hinterlegungsnummer: P35638
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DDIT3(1649)

Beschreibung

Allgemeine Beschreibung

DNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human GADD153/DDIT3/CHOP near the N-terminus.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
Apoptose - Weiterführende Produkte

This Anti-GADD153/DDIT3/CHOP antibody is validated for use in Western Blotting for the detection of GADD153/DDIT3/CHOP.

Qualität

Evaluated by Western Blotting in Tunicamycin treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD153/DDIT3/CHOP in 10 µg of Tunicamycin treated HeLa cell lysate.

Zielbeschreibung

~29 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable