AB5884

Anti-HCN1 Antibody

Chemicon^®, from rabbit

• Synonym(e): Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_021072.2
• UniProt-Hinterlegungsnummer: O60741
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HCN1(348980)

Beschreibung

Spezifität

Recognizes HCN-1 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1). The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse (18/19) and possibly rabbit (16/19) due to sequence homology. Other species have not been tested.

Immunogen

Synthetic peptide corresponding amino acids 6-24 of rat HCN-1 (Accession number AAF62173).

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Ionenkanäle & -transporter

Anti-HCN1 Antibody is an antibody against HCN1 for use in IH & WB.

Western blot: 1:200-1:400 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physikalische Form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Lagerung und Haltbarkeit

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• H-Sätze: H412
• P-Sätze: P273 - P501
• Gefahreneinstufungen: Aquatic Chronic 3
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3