AB5252P

Anti-Glial Derived Neurotrophic Factor Antibody

Chemicon^®, from sheep

• Synonym(e): GDNF
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: sheep
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, rat, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunohistochemistry: suitable
• NCBI-Hinterlegungsnummer: NM_000514.2; NM_199231.1; NM_199234.1
• UniProt-Hinterlegungsnummer: P39905
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GDNF(2668)

Beschreibung

Anwendung

Detect Glial Derived Neurotrophic Factor using this Anti-Glial Derived Neurotrophic Factor Antibody validated for use in IH.

Immunohistochemistry: 1 μg/mL

Immunoblotting: 1 μg/mL using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.

Optimal working dilutions must be determined by end user.

Physikalische Form

Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• H-Sätze: H412
• P-Sätze: P273 - P501
• Gefahreneinstufungen: Aquatic Chronic 3
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3