AB4332

Anti-acetyl-MVH (Lys405) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: pig, human, mouse
• Speziesreaktivität (Voraussage durch Homologie): porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
• Methode(n): dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_034159
• UniProt-Hinterlegungsnummer: Q61496
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: acetylation (Lys405)
• Angaben zum Gen: mouse ... Ddx4(13206)

Beschreibung

Allgemeine Beschreibung

MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.

Spezifität

This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.

Immunogen

Epitope: Acetylated Lys405

KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.

Anwendung

Research Category
Stammzellforschung

Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).

Qualität

Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.

Zielbeschreibung

76 kDa calculated

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Non-acetylated and acetylated MVH (Lys405)

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids