AB3046

Anti-Uncoupling Protein 3 Antibody

Chemicon^®, from rabbit

• Synonym(e): UCP3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_003356.2; NM_022803.1
• UniProt-Hinterlegungsnummer: P55916
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... UCP3(7352) ; rat ... Ucp3(25708)

Beschreibung

Allgemeine Beschreibung

Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein. It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Spezifität

Uncoupling Protein 3 (UCP3), human. It is expected that the antibody will recognize both the long and short forms (30-33 kDa & 24-27 kDa) however splice variants are expected and precise reactivity profile has yet to be determined. Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (Boss et al. 1997; Vidal-Puig et al. 1997). It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (Vidal-Puig et al. 1997). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Immunogen

14 amino acid peptide sequence (designated UCP32) mapping near the C-terminus of human UCP3 (Boss et al. 1997). The UCP32 peptide has no significant homology with UCP1 or UCP2.

Anwendung

Research Category
Stoffwechsel

Research Sub Category
Ionen- & Transportkanäle

Anti-Uncoupling Protein 3 Antibody is an antibody against Uncoupling Protein 3 for use in ELISA, IH & WB.

Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).

Optimal working dilutions must be determined by end user.

Zielbeschreibung

30-33 kDa & 24-27 kDa

Physikalische Form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Lagerung und Haltbarkeit

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable