8.55010

NovaSyn^®TGT alcohol resin

Novabiochem^®

• CAS-Nummer: 478242-36-3
• UNSPSC-Code: 13111023
• NACRES: NA.22

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: NovaSyn^® TG; Novabiochem^®
• Form: beads
• Eignung der Reaktion: reaction type: Fmoc solid-phase peptide synthesis
• Hersteller/Markenname: Novabiochem^®
• Anwendung(en): peptide synthesis
• Funktionelle Gruppe: alcohol
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

NovaSyn TGT alcohol resin is derived from TG amino resin by acylation with Bayer′s 4-carboxytrityl linker ^[1]. Before use the resin must be converted to the chloride form by heating with AcCl ^[2,3] or SOCl2 ^[4,5] in toluene. The unstable trityl chloride resin should then be used immediately. Attachment of α-amino acids is free from enantiomerization, making this support ideal for the immobilization of sensitive residues such as Cys and His. Iodine oxidation of peptides containing Cys(Trt) occurs with release of the peptide from the resin. This support is also ideally suited to the synthesis of prolyl peptides as the bulk of the trityl handle prohibits diketopiperazine formation ^[6].Cleavage of protected peptide acids from this matrix can be effected by treatment with AcOH/MeOH/DCM ^[1], 0.5% TFA or HFIP ^[7].

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins

Literature references

^[1] E. Bayer, et al. in ′Peptides, Chemistry, Structure & Biology, Proc. 13th American Peptide Symposium′, R. S. Hodges & J. A. Smith(Eds), ESCOM, Leiden, 1994, pp. 156.
^[2] J. M. J. Frechet, et al. (1975) Tetrahedron Lett., 3055.
^[3] T. M. Fyles, et al. (1976) Can. J. Chem., 54,935.
^[4] A. v. Vliet, et al. in ′Innovation & Perspectives in Solid Phase Synthesis, 2nd International Symposium′, R. Epton (Eds), Intercept UKLtd., Andover, 1992, pp. 475.
^[5] A. v. Vliet, et al. in ′Peptides 1992, Proc. 22nd European Peptide Symposium′, C. H. Schneider & A. N. Eberle (Eds), ESCOM, Leiden,1993, pp. 279.
^[6] G. Grübler, et al. in ′Innovation & Perspectives in Solid Phase Synthesis, 3rd International Symposium′, R. Epton (Eds), Mayflower Worldwide Ltd., Birmingham, 1994, pp. 517.
^[7] R. Bollhagen, et al. (1994) J. Chem. Soc., Chem. Commun., 2559.

Verlinkung

Replaces: 01-64-0066

Hinweis zur Analyse

Farbe (visuell): weiß bis gelb bis beige
Aussehen der Substanz (visuell): Kügelchen
Beladung (Photometrische Bestimmung des Fmoc-Chromophores nach Abspaltung vom Fmoc-Leu beladenen Harz.): 0.15 - 0.30 mmol/g
Aufquellvolumen (in CH₂Cl₂): chargenspezifischer Wert
Die Polymermatrix ist ein NovaSyn TG Harz (90 µm).

Rechtliche Hinweise

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable